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γδ T-Cells in
Chronic Myeloid Leukemia 

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γδ T-Cells in Endometriosis 

γδ T-Cells in Multiple Myeloma 

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γδ T-Cells in Glioblastoma 

γδ T-Cells in Multiple Myeloma 

γδ T-Cells in
CMV reactivation 

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Undruggable oncoproteins cMyc and NMyc bind to mediator of transcription with superior high affinity

Knight A, Houser J, Otasevic T, Juran V, Vybihal V, Smrcka M, Piskacek M

BioRxiv (2024) doi.org/10.1101/2024.03.15.585150

The NFkB activation domain is 14-amino-acid-long variant of the 9aaTAD

Houser J, Jendruchova K, Knight A and Piskacek M

Biochemical Journal (2023) doi.org/10.1042/BCJ20220605

Expansions of tumor reactive Vdelta1 gamma-delta T cells in newly diagnosed patients with chronic myeloid leukemia

Knight A, Piskacek M, Jurajda M, Prochazkova J, Racil Z, Zackova D, Mayer J

Cancer Immunology, Immunotherapy (2022) doi.org/10.1007/s00262-022-03312-3

Cryptic inhibitory regions nearby activation domains

Knight A and Piskacek M

Biochimie (2022) doi.org/10.1016/j.biochi.2022.05.004

Blocking of EphA2 on endometrial tumor cells reduces susceptibility to Vδ1 gamma-delta T cell-mediated killing

Hudecek R, Kohlova B, Siskova I, Piskacek M, Knight A

Frontiers in Immunology (2021) doi.org/10.3389/fimmu.2021.752646

Plasmacytoid dendritic cells in patients with MGUS and Multiple Myeloma

Knight A, Rihova L, Kralova R, Penka M, Adam Z, Pour L, Piskacek M and Hajek R

Journal of Clinical Medicine (2021) doi.org/10.3390/jcm10163717

The 9aaTAD activation domains in the four Yamanaka Oct4, Sox2, Myc, and Klf4 transcription factors

Piskacek M, Otasevic T, Repko M, Knight A

Stem Cell Rev and Rep (2021) doi.org/10.1007/s12015-021-10225-8   

Full version on www.biorxiv.org/content/10.1101/2019.12.15.876706v1.full.pdf

Universal two-point interaction of mediator KIX with 9aaTAD activation domains

Hofrova A, Lousa P, Kubickova M, Hritz J, Otasevic T, Repko M, Knight A and Piskacek M

Journal of Cellular Biochemistry (2021) doi.org/10.1002/jcb.30075

The evolution of the 9aaTAD domain in Sp2 proteins: inactivation with valines and intron reservoirs

Piskacek M, Havelka M, Jendruchova K, Knight A, Keegan LP

Cellular and Molecular Life Sciences (2019) doi.org/10.1007/s00018-019-03251-w

Nuclear Hormone Receptors: ancient 9aaTAD and evolutionally gained NCoA Activation Pathways

Piskacek M, Havelka M, Jendruchova K, Knight A

Journal of Steroid Biochemistry and Molecular Biology (2018) doi.org/10.1016/j.jsbmb.2018.11.008

The 9aaTAD is exclusive activation domain in Gal4

Piskacek M, Havelka M, Rezacova M, Knight A

PlosOne (2017) doi.org/10.1371/journal.pone.0169261

Gal4 activation domain 9aaTAD could be inactivated by adjacent mini-inhibitory domain

and reactivated by distal re-activation domain

Piskacek M, Havelka M, Rezacova M, Knight A

BioRxiv (2017) doi.org/10.1101/110882

The 9aaTAD Transactivation Domains: from Gal4 to p53

Piskacek M, Havelka M, Rezacova M, Knight A

PlosOne (2016) doi.org/10.1371/journal.pone.0162842

Shared structural features of the 9aaTAD family in complex with CBP

Piskacek M, Vasku A, Hajek R and Knight A

Molecular BioSystems (2014) doi.org/10.1039/c4mb00672k

CMV-independent lysis of glioblastoma by ex vivo expanded/activated Vδ1+ γδ T cells.

Knight A, Arnouk H, Britt W, Gillespie GY, Cloud GA, Harkins L, Su Y, Lowdell MW, Lamb LS

PLoS One (2013)​ doi.org/journal.pone.0068729

Human Vdelta1 gamma-delta T cells exert potent specific cytotoxicity against primary multiple myeloma cells.

Knight A, Mackinnon S, Lowdell MW

Cytotherapy (2012)​ doi.org/10.3109/14653249.2012.700766

The role of Vδ2-negative γδ T cells during cytomegalovirus reactivation in recipients of allogeneic stem cell transplantation.

Knight A, Madrigal AJ, Grace S, Sivakumaran J, Kottaridis P, Mackinnon S, Travers PJ, Lowdell MW

Blood (2010)​ doi.org/10.1182/blood-2010-01-255166

Rsp5 promotes Gene Activation mediated by 9aaTAD Transcription Factors Oaf1 and Gal4

Lipp J, Koranda M, Piskacek M 

Nature Precedings (2009) doi.org/10.1038/npre.2009.3968.1

A novel 9-amino-acid transactivation domain in the C-terminal part of Sox18

Sandholzer J, Hoeth M, Piskacek M, Mayer H, de Martin R

Biochem Biophys Res Commun (2007)​ doi.org/10.1016/j.bbrc.2007.06.095

9aaTAD Prediction result (2006)

Piskacek M

Nature Precedings (2009)​ doi.org/10.1038/npre.2009.3984.1

Nine-amino-acid transactivation domain: Establishment and prediction utilities
Piskacek S, Gregor M, Nemethova M, Grabner M, Kovarik P, Piskacek M
Genomics
(2007)​ doi.org/10.1016/j.ygeno.2007.02.003
 

Functional analysis of the Zn(2)Cys(6) transcription factors Oaf1p and Pip2p
Baumgartner U, Hamilton B, Piskacek M, Ruis H, Rottensteiner H
J Biol Chem
(1999)​ doi.org/10.1074/jbc.274.32.22208

Collaboration:

Predicting the function and subcellular location of Caenorhabditis elegans proteins

Gurvitz A, Langer S, Piskacek M, Hamilton B, Ruis H, Hartig A

Yeast (2000)​ doi.org/10.1002/1097-0061(20000930)17:3<188::AID-YEA27>3.0.CO;2-E

 

Conditional Knock-down of the Mitochondrial Magnesium Channel hMrs2

Piskacek M, Zotova L, Zsurka G and Schweyen RJ

Journal of Cellular and Molecular Medicine (2009) doi.org/10.1111/j.1582-4934.2008.00328.x 

​Functionally suppressive CD8 T regulatory cells are increased in patients

with multiple myeloma: a cause for immune impairment
Muthu Raja KR, Kubiczkova L, Rihova L, Piskacek M, Vsianska P, Hezova R, Pour L, Hajek R
PLoS One
(2012)​ doi.org/10.1371/journal.pone.0049446

Do human B-lymphocytes avoid aging until 60 years?

Knight A, Nemec P, Bretzova S, Valkova L, Kolmanova K, Vytopilova R, Havelka M,

Vsianska P, Rihova L, Krejci M and Piskacek M

Oncotarget (2016) doi.org/10.18632/oncotarget.10146

The inositol phosphatase SHIP2 enables sustained ERK activation downstream of FGF receptors by recruiting Src kinases

Fafilek B, Balek L, Bosakova MK, Varecha M, Nita A, Gregor T, Gudernova I, Krenova J, Ghosh S, Piskacek M,

Jonatova L, Cernohorsky NH, Zieba JT, Kostas M, Haugsten EM, Wesche J, Erneux C, Trantirek L, Krakow D, Krejci P.

Sci Signal (2018) doi.org/10.1126/scisignal.aap8608

Clozapine Reverses Dysfunction of Glutamatergic Neurons Derived From Clozapine-Responsive Schizophrenia Patients.

Hribkova H, Svoboda O, Bartecku E, Zelinkova J, Horinkova J, Lacinova L, Piskacek M, Lipovy B, Provaznik I, Glover JC, Kasparek T, Sun YM.
Front Cell Neurosci. (2022) doi: 10.3389/fncel.2022.830757

 

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